Spirillum nitrogen-fixing medium (ATCC® medium 838)
KH2PO4: 0.4 g
K2HPO4: 0.1 g
MgSO4 • 7H2O: 0.2 g
NaCl: 0.1 g
CaCl2: 0.02 g
FeCl3: 0.01 g
Na2MoO4 • 2H2O: 0.002 g
Sodium malate: 5.0 g
Yeast Extract: 0.05 g
Distilled water: 1.0 L

Adjust pH to 7.2-7.4
For semisolid medium, add 0.5 g of agar; for solid medium, add 15 g of agar. Autoclave at 121°C for 15 min.


Atlas R.M. Handbook of Microbiological Media, 3rd ed. – Boca Raton: CRC Press, 2004. – 2056 p.
Staff members
  • Professor Olga Turkovskaya, D. Sc. (Biol.), Head of Collection
  • Ekaterina Dubrovskaya, Ph.D. (Biol.), Curator
  • Anna Muratova, D. Sc. (Biol.), Bacteriologist
  • Sergey Golubev, Ph.D. (Biol.), Bacteriologist
  • Anastasia Bondarenkova, Ph.D. (Biol.), Bacteriologist