Modified AAM medium
K2HPO4: 2.0 g
KH2PO4: 6.0 g
MgSO4 • 7H2O: 0.2 g
NaCl: 0.1 g
CaCl2 • 2H2O: 0.026 g
Na2MoO4 • 2H2O: 0.002 g
FeCl3: 0.01 g
NH4Cl: 1.0 g
Fe(III) EDTA (1.64%): 4.0 ml
Distilled water: 1.0 L

Adjust pH to 6.5
For semisolid medium, add 0.5 g of agar and omit NH4Cl; for solid medium, add 15 g of agar. Autoclave at 121°C for 30 min.

The carbon sources are sterilized separately (i.e., DL-sodium malate) or filter sterilized (i.e., sucrose) and added aseptically to the minimal medium to give a final concentration of 0.03 M.

Hartmann A., Baldani J.I. The genus Azospirillum // The Prokaryotes, V. 5: Proteobacteria Alpha and Beta Subclasses // Eds. M. Dworkin, S. Falkow, E. Rosenberg, K.H. Schleifer, E. Stackebrandt. – Springer Verlag, New York, USA, 2006. P. 115–140.
Staff members
  • Professor Olga Turkovskaya, D. Sc. (Biol.), Head of Collection
  • Ekaterina Dubrovskaya, Ph.D. (Biol.), Curator
  • Anna Muratova, D. Sc. (Biol.), Bacteriologist
  • Sergey Golubev, Ph.D. (Biol.), Bacteriologist
  • Anastasia Bondarenkova, Ph.D. (Biol.), Bacteriologist