- Professor Olga Turkovskaya, D. Sc. (Biol.), Head of Collection
- Ekaterina Dubrovskaya, Ph.D. (Biol.), Curator
- Anna Muratova, D. Sc. (Biol.), Bacteriologist
- Sergey Golubev, Ph.D. (Biol.), Bacteriologist
- Anastasia Bondarenkova, Ph.D. (Biol.), Bacteriologist
Nfb medium
DL-malic acid: 5.0 g
K2HPO4: 0.5 g
MgSO4 • 7H2O: 0.2 g
NaCl: 0.1 g
CaCl2 • 2H2O: 0.02 g
Micronutrient solution: 2 ml
Bromthymol blue solution (0.5% in 0.2N KOH): 2 ml
Fe(III) EDTA (1.64%): 4.0 ml
Vitamin solution: 1.0 ml
Distilled water: 1.0 L
Adjust pH to 6.8
For semisolid medium, add 0.5 g of agar; for solid medium, add 15 g of agar. Autoclave at 121°C for 15 min.
Micronutrient solution:
CuSO4 • 5H2O: 0.4 g
ZnSO4 • 7H2O: 0.12 g
H3BO3: 1.4 g
Na2MoO4 • 2H2O: 1.0 g
MnSO4 • H2O: 1.5 g
Distilled water: 1.0 L
Vitamin solution:
Biotin: 10 mg
Pyridoxol HCL: 20 mg
Distilled water: 0.1 L
Hartmann A., Baldani J.I. The genus Azospirillum // The Prokaryotes, V. 5: Proteobacteria Alpha and Beta Subclasses // Eds. M. Dworkin, S. Falkow, E. Rosenberg, K.H. Schleifer, E. Stackebrandt. – Springer Verlag, New York, USA, 2006. P. 115–140.
K2HPO4: 0.5 g
MgSO4 • 7H2O: 0.2 g
NaCl: 0.1 g
CaCl2 • 2H2O: 0.02 g
Micronutrient solution: 2 ml
Bromthymol blue solution (0.5% in 0.2N KOH): 2 ml
Fe(III) EDTA (1.64%): 4.0 ml
Vitamin solution: 1.0 ml
Distilled water: 1.0 L
Adjust pH to 6.8
For semisolid medium, add 0.5 g of agar; for solid medium, add 15 g of agar. Autoclave at 121°C for 15 min.
Micronutrient solution:
CuSO4 • 5H2O: 0.4 g
ZnSO4 • 7H2O: 0.12 g
H3BO3: 1.4 g
Na2MoO4 • 2H2O: 1.0 g
MnSO4 • H2O: 1.5 g
Distilled water: 1.0 L
Vitamin solution:
Biotin: 10 mg
Pyridoxol HCL: 20 mg
Distilled water: 0.1 L
Hartmann A., Baldani J.I. The genus Azospirillum // The Prokaryotes, V. 5: Proteobacteria Alpha and Beta Subclasses // Eds. M. Dworkin, S. Falkow, E. Rosenberg, K.H. Schleifer, E. Stackebrandt. – Springer Verlag, New York, USA, 2006. P. 115–140.
Information